Mouse IGFBP2 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 84092-1-RR (same clone as 84092-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84092-1-RR (IGFBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84092-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01011-1

  Sandwich ELISA standard curve of MP01011-1, Mouse IGFBP2 Recombinant Matched Antibody Pair - PBS only. 84092-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1196. 84092-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP01011-1

  One mouse kidney was cultured for 18 hours in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were stimulated with 10 μg/mL of PHA and 10 ng/mL of PMA. An aliquot of the cell culture supernatant was removed, assayed for mouse IGFBP2 and measured 38.18 ng/mL.

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  Sample test of MP01011-1

  Serum of six mouse was measured. The IGFBP2 concentration of detected samples was determined to be 152.26 ng/mL with a range of 95.84-279.86 ng/mL.

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  Cytometric bead array standard curve of MP01011-1

  Cytometric bead array standard curve of MP01011-1, MOUSE IGFBP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84092-1-PBS. Detection antibody: 84092-2-PBS. Standard: Eg1196. Range: 1.563-100 ng/mL.

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  Affinity and Kinetic Characterization of 84092-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84092-1-RR against Mouse IGFBP2 were performed. The affinity constant is 0.571 nM.

IGFBP2 {ptg:Brand} Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84092-1-PBS

Clone No.241363D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

mIGFBP-2, Insulin-like growth factor-binding protein 2, IGFBP-2, IGF-binding protein 2, IBP-2

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Product Information

84092-1-PBS targets IGFBP2 as part of a matched antibody pair:

MP01011-1: 84092-1-PBS capture and 84092-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: insulin-like growth factor binding protein 2
• Calculated Molecular Weight: 33kDa
• Observed Molecular Weight: 33-35 kDa
• GenBank Accession Number: NM_008342.3
• Gene Symbol: Igfbp2
• Gene ID (NCBI): 16008
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P47877
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

IGFBP2 is a key member of the IGFBP family that is highly expressed in embryonic and fetal tissues and decreases significantly after birth. IGFBP2 mediates IGF-independent tumorigenesis by participating in intracellular and nuclear regulatory networks and may serve as an excellent prognostic biomarker in various cancers (PMID: 35546443).