CoraLite® Plus 488-conjugated IGFBP2 Recombinant monoclonal antibody

• 

  IF Staining of HeLa using CL488-84092-3

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 IGFBP2 antibody (CL488-84092-3, Clone: 241363D8 ) at dilution of 1:200, CL594-Phalloidin (red).

IGFBP2 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-84092-3

Clone No.241363D8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC

Synonyms

IBP-2, IGF-binding protein 2, IGFBP-2, Insulin-like growth factor-binding protein 2, mIGFBP-2

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84092-3 targets IGFBP2 in IF/ICC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1196

  Product name: Recombinant Mouse IGFBP2 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 35-305 aa of NM_008342.3

  Sequence: EVLFRCPPCTPERLAACGPPPDAPCAELVREPGCGCCSVCARQEGEACGVYIPRCAQTLRCYPNPGSELPLKALVTGAGTCEKRRVGTTPQQVADSDDDHSEGGLVENHVDGTMNMLGGGSSAGRKPLKSGMKELAVFREKVNEQHRQMGKGAKHLSLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDDRGPLEHLYSLHIPNCDKHGRYNLKQCKMSLNGQRGECWCVNPNTGKPIQGAPTIRGDPECHLFYNEQQETGGAHAQSVQ

• Full Name: insulin-like growth factor binding protein 2
• Calculated Molecular Weight: 33kDa
• GenBank Accession Number: NM_008342.3
• Gene Symbol: Igfbp2
• Gene ID (NCBI): 16008
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P47877
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IGFBP2 is a key member of the IGFBP family that is highly expressed in embryonic and fetal tissues and decreases significantly after birth. IGFBP2 mediates IGF-independent tumorigenesis by participating in intracellular and nuclear regulatory networks and may serve as an excellent prognostic biomarker in various cancers (PMID: 35546443).