CoraLite®594-conjugated IgD Polyclonal antibody

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  IF Staining of human tonsillitis using CL594-16489

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 human IgD antibody (CL594-16489) at dilution of 1:200.

human IgD Polyclonal Antibody for IF-P

Cat No. CL594-16489

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P

Synonyms

FLJ00382, FLJ46727, IGHD

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-16489 targets human IgD in IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9614

  Product name: Recombinant human IGHD protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 202-548 aa of BC021276

  Sequence: RRDSYYMTSSQLSTPLQQWRQGEYKCVVQHTASKSKKEIFRWPESPKAQASSVPTAQPQAEGSLAKATTAPATTRNTGRGGEEKKKEKEKEEQEERETKTPECPSHTQPLGVYLLTPAVQDLWLRDKATFTCFVVGSDLKDAHLTWEVAGKVPTGGVEEGLLERHSNGSQSQHSRLTLPRSLWNAGTSVTCTLNHPSLPPQRLMALREPAAQAPVKLSLNLLASSDPPEAASWLLCEVSGFSPPNILLMWLEDQREVNTSGFAPARPPPQPGSTTFWAWSVLRVPAPPSPQPATYTCVVSHEDSRTLLNASRSLEVSYLAMTPLIPQSKDENSDDYTTFDDVGSLWT

• Full Name: immunoglobulin heavy constant delta
• Calculated Molecular Weight: 573 aa, 63 kDa
• GenBank Accession Number: BC021276
• Gene Symbol: IGHD
• Gene ID (NCBI): 3495
• RRID: AB_2919834
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. This antibody recognize the heavy chain (~60 kDa) of IgD.