Filter: AllWBIF/ICCAffinity

WB analysis using 86939-1-RR (same clone as 86939-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 86939-1-RR (IGJ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86939-1-PBS in a different storage buffer formulation.
IF Staining of Ramos using 86939-1-RR (same clone as 86939-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed Ramos cells using IGJ antibody (86939-1-RR, Clone: 251691G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86939-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 86939-1-RR
Biolayer interferometry (BLl) kinetic assays of 86939-1-RR against Human IGJ were performed. The affinity constant is below 1 pM.

IGJ Recombinant monoclonal antibody, PBS Only

Name: IGJ Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 86939-1-PBS

IGJ Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86939-1-PBS

Clone No.251691G5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

IGCJ, Immunoglobulin J chain, JCH, JCHAIN, Joining chain of multimeric IgA and IgM

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

86939-1-PBS targets IGJ in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag4559 Product name: Recombinant human IGJ protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-159 aa of BC038982 Sequence: MKNHLLFWGVLAVFIKAVHVKAQEDERIVLVDNKCKCARITSRIIRSSEDPNEDIVERNIRIIVPLNNRENISDPTSPLRTRFVYHLSDLCKKCDPTEVELDNQIVTATQSNICDEDSATETCYTYDRNKCYTAVVPLVYGGETKMVETALTPDACYPD Predict reactive species
Full Name	immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides
Calculated Molecular Weight	158 aa, 18 kDa
Observed Molecular Weight	25 kDa
GenBank Accession Number	BC038982
Gene Symbol	IGJ
Gene ID (NCBI)	3512
RRID	AB_3745221
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P01591
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

IGJ, the immunoglobulin J chain, serves to link two monomer units of either IgM or IgA.

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