IGJ Recombinant monoclonal antibody

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  WB analysis using 86939-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86939-1-RR (IGJ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Ramos using 86939-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Ramos cells using IGJ antibody (86939-1-RR, Clone: 251691G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86939-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86939-1-RR against Human IGJ were performed. The affinity constant is below 1 pM.

IGJ Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86939-1-RR

Clone No.251691G5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

IGCJ, Immunoglobulin J chain, JCH, JCHAIN, Joining chain of multimeric IgA and IgM

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, Ramos cells, human plasma
• Positive IF/ICC detected in: Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86939-1-RR targets IGJ in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4559

  Product name: Recombinant human IGJ protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-159 aa of BC038982

  Sequence: MKNHLLFWGVLAVFIKAVHVKAQEDERIVLVDNKCKCARITSRIIRSSEDPNEDIVERNIRIIVPLNNRENISDPTSPLRTRFVYHLSDLCKKCDPTEVELDNQIVTATQSNICDEDSATETCYTYDRNKCYTAVVPLVYGGETKMVETALTPDACYPD

• Full Name: immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides
• Calculated Molecular Weight: 158 aa, 18 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC038982
• Gene Symbol: IGJ
• Gene ID (NCBI): 3512
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01591
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGJ, the immunoglobulin J chain, serves to link two monomer units of either IgM or IgA.