Ig Kappa Light Chain Polyclonal antibody

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• Ig Kappa Light Chain Polyclonal antibody (14678-1-AP)

Ig Kappa Light Chain Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14678-1-AP Copied!

Publications (24)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA

Synonyms

IgG Kappa Light Chain, IGK, IGK-, IGKC, immunoglobulin kappa locus

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  WB analysis using 14678-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14678-1-AP (Ig Kappa Light Chain antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human plasma using 14678-1-AP

  human plasma (0.3ug) were subjected to SDS PAGE followed by western blot with 14678-1-AP (IgG light chain (Kappa) Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skeletal muscle using 14678-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 14678-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human spleen using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human spleen using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse spleen using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse spleen using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human plasma tissue, mouse skeletal muscle tissue, human liver tissue, human plasma, Raji cells
• Positive IHC detected in: human spleen tissue, human heart tissue, human placenta tissue, human skin tissue, human testis tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14678-1-AP targets Ig Kappa Light Chain in WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6389

  Product name: Recombinant human IGK- protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-234 aa of BC030813

  Sequence: MEAPAQLLFLLLLWLPDTTGEIVMTQSPATLSVSPGERATLSCRASQSVTSNLAWYQQTPGQSPRLVIYGASSRASGVPARFSGSGSGTEFTLTISSLQSEDFAVYYCQQYNKWPHTFGQGTKLDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

• Full Name: immunoglobulin kappa locus
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC030813
• Gene Symbol: IgG Kappa Light Chain
• Gene ID (NCBI): 50802
• RRID: AB_1851235
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detects the light chain (Kappa) of human immunoglobulin.

Publications

What published studies show

The Ig Kappa Light Chain Polyclonal antibody (Cat# 14678-1-AP) has 24 citations. These appear in journals including Cancer Cell, Protein Cell, Nature Communications, Biomarker Research, Journal of Experimental & Clinical Cancer Research, EBioMedicine, Food Research International, Cell Mol Gastroenterol Hepatol, Ocular Surface, Cancer Gene Therapy, Translational Research, Cell Biology Toxicology, Noncoding RNA Research, Molecular Medicine Reports, Molecular & Cellular Biochemistry, FASEB Journal, PLoS Genetics, Journal of Immunology Research, Journal of Bone & Mineral Metabolism, PeerJ, Turkish Journal of Gastroenterology, Journal of Toxicological Sciences, and Heliyon. Research fields span oncology (colorectal, lung, liver, pancreatic, head & neck cancers), immunology, RNA/m6A modification, fibrosis, and inflammation.

Automatically generated from the 24 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Cancer Cell; CCL19-producing fibroblasts promote tertiary lymphoid structure formation enhancing anti-tumor IgG response in colorectal cancer liver metastasis; Authors - Yifan Zhang
human	IHC	Cancer Cell; Spatial transcriptomics reveals tryptophan metabolism restricting maturation of intratumoral tertiary lymphoid structures; Authors - Zhonghui Tang
	IF	Protein Cell; Single-nucleus transcriptomics uncovers a geroprotective role of YAP in primate gingival aging; Authors - Qinchao Hu
	IHC	Nat Commun; A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice.; Authors - Christine Quach
		Biomark Res; NPC1 as a novel therapeutic target for induction of pyroptosis in cancers; Authors - Chuanchao Zhang
	RIP	J Exp Clin Cancer Res; RNA N6-methyladenosine reader IGF2BP2 promotes lymphatic metastasis and epithelial-mesenchymal transition of head and neck squamous carcinoma cells via stabilizing slug mRNA in an m6A-dependent manner.; Authors - Dan Yu