IGSF2 Polyclonal antibody

IGSF2 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26047-1-AP

Synonyms

CD101, Cell surface glycoprotein V7, EWI 101, EWI101, IGSF2, V7

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Validation Data Gallery

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  WB analysis using 26047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26047-1-AP (IGSF2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 26047-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26047-1-AP (IGSF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 26047-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26047-1-AP (IGSF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 26047-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26047-1-AP (IGSF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 26047-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed RAW 264.7 cells using 26047-1-AP (IGSF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of RAW 264.7 using 26047-1-AP

  1X10^6 RAW 264.7 cells were stained with 0.2ug IGSF2 antibody (26047-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: HCT 116 cells, RAW 264.7 cells, Jurkat cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: RAW 264.7 cells
• Positive FC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of 26047-1-AP is IGSF2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGSF2 fusion protein Ag23297
• Full Name: immunoglobulin superfamily, member 2
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC136484
• Gene Symbol: IGSF2
• Gene ID (NCBI): 9398
• RRID: AB_2880350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGSF2, also known as CD101, is a low abundance protein. Expression of CD101 is found on monocytes, granulocytes and dendritic cells. In addition expression on T lymphocytes is important for cell activation. It plays a role as inhibitor of T-cells proliferation induced by CD3 and inhibits expression of IL2RA on activated T-cells and secretion of IL2. This antibody specifically recognises the 115 kDa protein.

Publications

Species	Application	Title
human	IF	Front Immunol; Identification of CD101 in Glioma: A Novel Prognostic Indicator Expressed on M2 Macrophages.; Authors - Yuyang Liu