IHCeasy PHKB Ready-To-Use IHC Kit
PHKB Ready-to-use reagent kit for IHC.
Cat no : KHC2772
Synonyms
Phosphorylase kinase subunit beta
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Product Information
KHC2772 is a ready-to-use IHC kit for staining of PHKB. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy PHKB Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
PHKB gene encodes phosphorylase kinase subunit beta involved in glycan biosynthesis and glycogen metabolism. PHKB activity is regulated by phosphorylation of various serine residues, and catalyzes the phosphorylation of serine in certain substrates, including troponin I. Phosphorylase kinase (PhK) complex, composed of alpha, beta, gamma, and delta subunits, stimulates energy production from glycogen in the cascade activation of glycogenolysis. Its large homologous alpha and beta subunits regulate the activity of the catalytic gamma subunit. Defects in PHKB are the cause of glycogen storage disease type 9B (GSD9B) also known as phosphorylase kinase deficiency of liver and muscle (PKD), characterized by hepathomegaly, only slightly elevated transaminases and plasma lipids, clinical improvement with increasing age, and remarkably no clinical muscle involvement.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | Phosphorylase kinase subunit beta |