IK Polyclonal antibody, PBS Only

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• IK Polyclonal antibody, PBS Only (31833-1-PBS)

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  WB analysis using 31833-1-AP (same clone as 31833-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31833-1-AP (IK antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31833-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 31833-1-AP (same clone as 31833-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31833-1-AP (IK antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31833-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 31833-1-AP (same clone as 31833-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using IK antibody (31833-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 31833-1-PBS in a different storage buffer formulation.

IK Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 31833-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

RED, Protein RER, Protein Red, IK factor, IK Cytokine

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Product Information

31833-1-PBS targets IK in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag37095

  Product name: Recombinant human IK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-216 aa of BC013005

  Sequence: MPERDSEPFSNPLAPDGHDVDDPHSFHQSKLTNEDFRKLLMTPRAAPTSAPPSKSRHHEMPREYNEDEDPAARRRKKKSYYAKLRQQEIERERELAEKYRDRAKERRDGVNKDYEETELISTTANYRAVGPTAEADKSAAEKRRQLIQESKFLGGDMEHTHLVKGLDFALLQKVRAEIASKEKEEEELMEKPQKETKKDEDPENKIEFKTRLGRNV

• Full Name: IK cytokine, down-regulator of HLA II
• Calculated Molecular Weight: 557 aa, 66 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC013005
• Gene Symbol: IK
• Gene ID (NCBI): 3550
• RRID: AB_3670123
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q13123
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IK, also known as the nuclear protein RED, is a down-regulator of human leukocyte antigen (HLA) II and interacts with the human SAC protein MAD1, which is required for the localization of the kinetochore of MAD1, for the progression of mitosis and for the activation of the SAC. IK is therefore an important protein for the correct progression of mitosis through the regulation of mitotic kinases and phosphatases. In addition, IK may be involved in pre-mRNA splicing, which may be a useful biomarker for new nuclear structures in cells.