Tested Applications
| Positive WB detected in | LPS and Brefeldin A treated THP-1 cells, A431 cells, HT-1080 cells, U-87 MG cells |
| Positive IF/ICC detected in | LPS and Brefeldin A treated THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82696-15-RR targets IL-1 beta in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg0286 Product name: Recombinant Human IL-1 Beta protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: N-6*HIS Domain: 117-269 aa of BC008678 Sequence: APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS Predict reactive species |
| Full Name | interleukin 1, beta |
| Calculated Molecular Weight | 269 aa, 31 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC008678 |
| Gene Symbol | IL1B |
| Gene ID (NCBI) | 3553 |
| ENSEMBL Gene ID | ENSG00000125538 |
| RRID | AB_3670529 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01584 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IL-1 beta antibody 82696-15-RR | Download protocol |
| WB protocol for IL-1 beta antibody 82696-15-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IL-1 beta Recombinant monoclonal antibody (Cat# 82696-15-RR) has 3 citations. They appear in Molecular Medicine Reports, Food Science & Nutrition, and Animal Nutrition. Research fields include intestinal ischemia-reperfusion injury and oxidative stress, wound healing via angiogenesis and ferroptosis inhibition, and dietary fatty acid effects on sow reproductive performance and piglet intestinal health. All cited applications are Western blot in human samples, except the animal nutrition study.
| Species | Application | Title |
|---|---|---|
Food Sci Nutr Resveratrol Promotes Wound Healing by Enhancing Angiogenesis via Inhibition of Ferroptosis | ||
Mol Med Rep CTSB mediates oxidative stress and intestinal epithelial barrier disruption in intestinal ischemia‑reperfusion injury. | ||
Anim Nutr Effects of dietary supplementation with fat powder with different n-6/n-3 polyunsaturated fatty acid ratios on the reproductive performance of sows and intestinal health of piglets. |
