Anti-Mouse IL-1 alpha Rabbit Recombinant Antibody

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  FC experiment of RAW 264.7 using 98052-1-RR

  1x10^6 LPS treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL1-alpha Rabbit Recombinant Antibody (98052-1-RR, Clone:240431G10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of RAW 264.7 using 98052-1-RR

  1x10^6 LPS treated (red) or untreated (black) RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL1-alpha Rabbit Recombinant Antibody (98052-1-RR, Clone: 240431G10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 98052-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98052-1-RR against Mouse IL-1 alpha were performed. The affinity constant is 0.495 nM.

IL-1 alpha {ptg:Brand} Recombinant Antibody for FC (Intra)

Cat No. 98052-1-RR

Clone No.240431G10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

IL1-alpha, Il1a, IL-1,IL1, IL1 alpha, Il 1a

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Tested Applications

• Positive FC (Intra) detected in: LPS treated RAW 264.7 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98052-1-RR targets IL-1 alpha in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 1 alpha
• Calculated Molecular Weight: 31kd
• GenBank Accession Number: NM_010554
• Gene Symbol: Il1a
• Gene ID (NCBI): 16175
• RRID: AB_3672199
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P01582
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

IL-1 alpha is a dual function cytokine that can function both as a transcription factor and a damage-associated molecular pattern (DAMP) in innate immune responses. It is expressed by many cell types and its expression is increased when cells are exposed to growth factors, pro-inflammatory, and stress-related signals. In its pro-form, IL-1 alpha contains a nuclear localization signal that allows it to translocate to the nucleus and bind to DNA, resulting in either the increase or prohibition of pro-inflammatory cytokine expression. When cells are exposed to necrotic signals, mature IL-1 alpha is released from the cell lysates and triggers a strong pro-inflammatory response upon binding to its receptor on nearby cells. Due to its strongly pro-inflammatory nature, IL-1 alpha has been linked to tumor metastasis and lower median survival in several cancers (PMID: 27434011, 29247995).