FITC Plus Anti-Human IL-10 (7B8G3)

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  FC experiment of human PBMCs using FITC-65286

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug FITC Plus Anti-Human IL-10 (FITC-65286, Clone:7B8G3) or Mouse IgG2b Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65286

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug FITC Plus Anti-Human IL-10 (FITC-65286, Clone:7B8G3) (red) or Mouse IgG2b Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug FITC Plus Anti-Human IL-10 (FITC-65286, Clone:7B8G3) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

IL-10 Monoclonal Antibody for FC (Intra)

Cat No. FITC-65286

Clone No.7B8G3

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC (Intra)

Synonyms

IL-10, IL-10,IL10, IL10A, interleukin 10, Interleukin-10, TGIF, 7B8G3, CSIF, IL 10, IL10

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Tested Applications

• Positive FC (Intra) detected in: LPS and Brefeldin A treated human PBMCs
• Positive FC detected in: LPS and Brefeldin A treated human PBMCs

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension
• Flow Cytometry (FC): FC :

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65286 targets IL-10 in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-10 fusion protein HZ-1145
• Full Name: interleukin 10
• Calculated Molecular Weight: 21 kDa
• GenBank Accession Number: BC104252
• Gene Symbol: IL-10
• Gene ID (NCBI): 3586
• RRID: AB_3085009
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.