Anti-Human IL-12A/IL-12 p35 (1E12H6), PBS Only

• 

  FC experiment of human PBMCs using 65277-1-Ig (same clone as 65277-1-PBS)

  1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with CoraLite ® Plus 750 Anti-Human HLA-DR then intracellularly stained with 0.2 ug CoraLite ® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65277-1-Ig. This data was developed using the same antibody clone with 65277-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65277-1-Ig (same clone as 65277-1-PBS)

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) (red) or Mouse IgG1 Isotype Control (blue), or unstimulated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65277-1-Ig. This data was developed using the same antibody clone with 65277-1-PBS in a different storage buffer formulation.

IL-12A/IL-12 p35 Monoclonal Antibody for FC (Intra)

Cat No. 65277-1-PBS

Clone No.1E12H6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Synonyms

IL-12, 1E12H6, CLMF, CLMF p35, IL 12 subunit p35

---

Product Information

65277-1-PBS targets IL-12A/IL-12 p35 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: BC104982
• Gene Symbol: IL-12A
• Gene ID (NCBI): 3592
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P29459
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: -20°C.

Background Information

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).