FcZero-rAb® Biotin Anti-Human IL-12RB1/CD212 Rabbit Recombinant Antibody

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• FcZero-rAb® Biotin Anti-Human IL-12RB1/CD212 Rabbit Recombinant Antibody (Biotin-FcA98149)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

IL-12RB1/CD212 FcZero-rAb^® Recombinant Antibody for FC

Cat No. Biotin-FcA98149 Copied!

Clone No. 241661H1

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

IL12RB1, CD212, IL 12R beta 1, IL 12R subunit beta 1, IL 12RB1

• 

  FC experiment of human PBMCs using Biotin-FcA98149

  1x10^6 anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Biotin Anti-Human IL-12RB1/CD212 Rabbit RecAb (Biotin-FcA98149, Clone: 241661H1) (red) or Isotype Control (blue), and Streptavidin-PE Conjugate (PE-PF00030). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Biotin Anti-Human IL-12RB1/CD212 Rabbit RecAb (Biotin-FcA98149, Clone: 241661H1) (black, dashed), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: anti-CD3/CD28 treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98149 targets IL-12RB1/CD212 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: interleukin 12 receptor, beta 1
• Calculated Molecular Weight: 662 aa, 73 kDa
• GenBank Accession Number: BC029121
• Gene Symbol: IL12RB1
• Gene ID (NCBI): 3594
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42701
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin-12 receptor subunit beta-1 (IL-12RB1), also named as CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. IL-12RB1 localizes in the membrane and functions as an interleukin receptor which binds IL-12 with low affinity and is involved in IL-12 transduction. Associated with IL-12RB2 it forms a functional, high affinity receptor for IL-12. It can also associate with IL23R to form the interleukin-23 receptor which functions in IL-23 signal transduction probably through activation of the Jak-Stat signaling cascade.