FcZero-rAb® Biotin Anti-Human IL-13Ra1/CD213a1 Rabbit Recombinant Antibody

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• FcZero-rAb® Biotin Anti-Human IL-13Ra1/CD213a1 Rabbit Recombinant Antibody (Biotin-FcA98216)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

IL-13Ra1/CD213a1 FcZero-rAb^® Recombinant Antibody for FC, ELISA

Cat No. Biotin-FcA98216 Copied!

Clone No. 241834A1

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, ELISA

Synonyms

IL13RA1, Cancer/testis antigen 19, CD213A1, CT19, IL 13 receptor subunit alpha 1

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  FC experiment of MCF-7 using Biotin-FcA98216

  1x10^6 MCF-7 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Biotin Anti-Human IL-13Ra1/CD213a1 Rabbit RecAb (Biotin-FcA98216, Clone: 241834A1) (red) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (Cat.No. PE-PF00030). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: MCF-7 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98216 targets IL-13Ra1/CD213a1 in FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2320

  Product name: Recombinant Human IL-13Ra1 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 22-343 aa of BC009960

  Sequence: GGGGAAPTETQPPVTNLSVSVENLCTVIWTWNPPEGASSNCSLWYFSHFGDKQDKKIAPETRRSIEVPLNERICLQVGSQCSTNESEKPSILVEKCISPPEGDPESAVTELQCIWHNLSYMKCSWLPGRNTSPDTNYTLYYWHRSLEKIHQCENIFREGQYFGCSFDLTKVKDSSFEQHSVQIMVKDNAGKIKPSFNIVPLTSRVKPDPPHIKNLSFHNDDLYVQWENPQNFISRCLFYEVEVNNSQTETHNVFYVQEAKCENPEFERNVENTSCFMVPGVLPDTLNTVRIRVKTNKLCYEDDKLWSNWSQEMSIGKKRNST

• Full Name: interleukin 13 receptor, alpha 1
• Calculated Molecular Weight: 49 kDa
• GenBank Accession Number: BC009960
• Gene Symbol: IL-13Rα1
• Gene ID (NCBI): 3597
• RRID: AB_3746977
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78552
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

IL-13 is an immunoregulatory cytokine secreted predominantly by activated T-helper 2 (Th2) cells and performs multiple functions through a complex receptor system comprised of IL-4Rα and two IL-13 binding proteins, IL-13Rα1 and IL-13Rα2 (PMID: 12704343). IL-13Rα1 is a glycosylated transmembrane protein (PMID: 10856136; 9723655). IL-13Rα1 is expressed on a wide range of cells including B cells, monocytes, macrophages, basophils, mast cells, and endothelial cells (PMID: 10856136; 31866859). IL-13 binding to the IL-4Rα/IL-13Rα1 complex leads to activation of JAK1, JAK2, and TYK2 and subsequent phosphorylation of STAT6 and STAT3 (PMID: 31866859).