IL-15RA Recombinant antibody

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  WB analysis using 84582-8-RR

  Human placenta tissue was subjected to SDS PAGE followed by western blot with 84582-8-RR (IL15RA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

IL-15RA Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84582-8-RR

Clone No.242689D6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IL15RA, CD215, IL 15 receptor subunit alpha, IL 15R alpha, IL 15RA

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Tested Applications

• Positive WB detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84582-8-RR targets IL-15RA in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 15 receptor, alpha
• Calculated Molecular Weight: 231 aa, 24 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC107777
• Gene Symbol: IL-15RA
• Gene ID (NCBI): 3601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13261
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897). The calculated molecular weight of full-length IL-15Rα is 28 kDa, IL-15Rα can be glycosylated, and larger apparent molecular weights have been reported by some researches (PMID: 10480910; 15976182; 15671076; 17912462).