PE Anti-Human IL-15RA Rabbit Recombinant Antibody

• Home
• Products
• Primary Antibodies
• Flow Cytometry Antibodies
• PE Anti-Human IL-15RA Rabbit Recombinant Antibody (PE-98380-3)

IL-15RA Uni-rAb^® Recombinant Antibody for FC

Cat No. PE-98380-3

Clone No.242689E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

IL15RA, CD215, IL 15 receptor subunit alpha, IL 15R alpha, IL 15RA

• 

  FC experiment of human PBMCs using PE-98380-3

  1x10^6 untreated or LPS and IFN-γ treated human PBMCs were surface stained with APC Anti-Human CD14 and 5 ul PE Anti-Human IL-15RA Rabbit RecAb (PE-98380-3, Clone:242689E1). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using PE-98380-3

  1x10^6 LPS and IFN-γ treated human PBMCs were surface stained with 5 ul PE Anti-Human IL15RA Rabbit RecAb (PE-98380-3, Clone: 242689E1) (red) or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul PE Anti-Human IL15RA Rabbit RecAb (PE-98380-3, Clone: 242689E1) (black, dashed). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive FC detected in: LPS and IFN-γ treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-98380-3 targets IL-15RA in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: interleukin 15 receptor, alpha
• Calculated Molecular Weight: 231 aa, 24 kDa
• GenBank Accession Number: BC107777
• Gene Symbol: IL-15RA
• Gene ID (NCBI): 3601
• RRID: AB_3749606
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Excitation Laser: Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13261
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897).