IL-16 Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis of Recombinant protein using 86927-2-RR (same clone as 86927-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86927-2-RR (IL-16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86927-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 86927-2-RR (same clone as 86927-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86927-2-RR (IL-16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86927-2-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 86927-2-RR (same clone as 86927-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using IL-16 antibody (86927-2-RR, Clone: 251661G11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86927-2-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02772-1

  Human peripheral blood mononuclear cells (PBMC) were cultured  unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean IL-16 concentration was determined to be 5.5 ng/mL in unstimulated PBMC supernatant,15.5 ng/mL in PHA stimulated PBMC supernatant.

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  Cytometric bead array standard curve of MP02772-1

  Cytometric bead array standard curve of MP02772-1, IL-16 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86927-2-PBS. Detection antibody: 86927-1-PBS. Standard: Eg4831. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 86927-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86927-2-RR against Human IL-16 were performed. The affinity constant is 0.94 nM.

IL-16 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 86927-2-PBS

Clone No.251661G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

IL16, IL 16, interleukin-16, prIL 16

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Product Information

86927-2-PBS targets IL-16 as part of a matched antibody pair:

MP02772-1: 86927-2-PBS capture and 86927-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg4831

  Product name: Recombinant Human IL-16 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 1-130 aa of AAC12732.1

  Sequence: MPDLNSSTDSAASASAASDVSVESTAEATVCTVTLEKMSAGLGFSLEGGKGSLHGDKPLTINRIFKGAASEQSETVQPGDEILQLGGTAMQGLTRFEAWNIIKALPDGPVTIVIRRKSLQSKETTAAGDS

• Full Name: interleukin 16 (lymphocyte chemoattractant factor)
• Calculated Molecular Weight: 9 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: AAC12732.1
• Gene Symbol: IL-16
• Gene ID (NCBI): 3603
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IL-16 is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. It is mainly produced by T lymphocytes but also by other immune cells, neuronal cells, fibroblasts and epithelial cells. The signaling process of this cytokine is mediated by CD4. IL-16 induces chemotaxis of CD4+ cells such as lymphocytes, eosinophils, and dendritic cells by ligating CD4 directly at a site distinct from other ligands. Among its multiple functions, IL-16 is a T cell chemoattractant involved in T helper cell inflammatory responses and the regulation of both T cell growth, and responsiveness to regulatory cytokines. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control.