IL-16 Recombinant monoclonal antibody

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  WB analysis of Recombinant protein using 86927-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86927-2-RR (IL-16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86927-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86927-2-RR (IL-16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 86927-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using IL-16 antibody (86927-2-RR, Clone: 251661G11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86927-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86927-2-RR against Human IL-16 were performed. The affinity constant is 0.94 nM.

IL-16 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86927-2-RR

Clone No.251661G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

IL16, IL 16, interleukin-16, prIL 16

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Tested Applications

• Positive WB detected in: Recombinant protein, Jurkat cells, HuT 78 cells
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86927-2-RR targets IL-16 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg4831

  Product name: Recombinant Human IL-16 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 1-130 aa of AAC12732.1

  Sequence: MPDLNSSTDSAASASAASDVSVESTAEATVCTVTLEKMSAGLGFSLEGGKGSLHGDKPLTINRIFKGAASEQSETVQPGDEILQLGGTAMQGLTRFEAWNIIKALPDGPVTIVIRRKSLQSKETTAAGDS

• Full Name: interleukin 16 (lymphocyte chemoattractant factor)
• Calculated Molecular Weight: 9 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: AAC12732.1
• Gene Symbol: IL-16
• Gene ID (NCBI): 3603
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-16 is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. It is mainly produced by T lymphocytes but also by other immune cells, neuronal cells, fibroblasts and epithelial cells. The signaling process of this cytokine is mediated by CD4. IL-16 induces chemotaxis of CD4+ cells such as lymphocytes, eosinophils, and dendritic cells by ligating CD4 directly at a site distinct from other ligands. Among its multiple functions, IL-16 is a T cell chemoattractant involved in T helper cell inflammatory responses and the regulation of both T cell growth, and responsiveness to regulatory cytokines. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control.