IL-18 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82907-1-RR (same clone as 82907-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82907-1-RR (IL-18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82907-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82907-1-RR (same clone as 82907-1-PBS)

  WB result of IL-18 antibody (82907-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-18 transfected HeLa cells. This data was developed using the same antibody clone with 82907-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 82907-1-RR (same clone as 82907-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82907-1-RR (IL-18 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82907-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 82907-1-RR (same clone as 82907-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82907-1-RR (IL-18 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82907-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82907-1-RR (same clone as 82907-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IL-18 antibody (82907-1-RR, Clone: 1H14 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82907-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 82907-1-RR (same clone as 82907-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug IL-18 Recombinant antibody (82907-1-RR, Clone:1H14) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82907-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82907-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82907-1-RR against Human IL-18 were performed. The affinity constant is 0.769 nM.

• Featured Product
• KD/KO Validated

IL-18 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 82907-1-PBS

Clone No.1H14

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

IL18, Pre-IL18, 1H14, Iboctadekin, IFN-gamma-inducing factor

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Product Information

82907-1-PBS targets IL-18 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1063

  Product name: Recombinant human Pre IL-18 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-193 aa of BC007461

  Sequence: MAAEPVEDNCINFVAMKFIDNTLYFIAEDDENLESDYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED

• Full Name: IL-18
• Calculated Molecular Weight: 193 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC007461
• Gene Symbol: IL-18
• Gene ID (NCBI): 3606
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14116
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IL-18 is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL-18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL-18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form.