IL-18 Recombinant antibody

IL-18 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H14

Cat no : 82907-1-RR

Synonyms

Iboctadekin, IFN gamma inducing factor, IGIF, IL 1 gamma, IL 18, IL 1g, IL18, IL-18, IL1F4, Interleukin 1 gamma, Interleukin 18

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Validation Data Gallery

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  WB analysis using 82907-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82907-1-RR (IL-18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, U2OS cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82907-1-RR targets IL-18 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IL-18 fusion protein Ag1063
• Full Name: IL-18
• Calculated Molecular Weight: 193 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC007461
• Gene Symbol: IL18
• Gene ID (NCBI): 3606
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-18 is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL-18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL-18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form.