FcZero-rAb™ APC Anti-Human IL-18R1/CD218a Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using APC-FcA98150

  1x10^6 human peripheral blood leukocytes were surface stained with FcZero-rAb™ PE Anti-Human NCAM1/CD56, and 5 ul APC Anti-Human IL-18R1/CD218a Rabbit RecAb (APC-FcA98150, Clone: 241673D3) or APC Rabbit IgG Isotype Control RecAb (APC-FcA98136, Clone: 240953C9). Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

IL-18R1/CD218a FcZero-rAb^TM Recombinant Antibody for FC

Cat No. APC-FcA98150

Clone No.241673D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

IL18R1, CD218 antigen-like family member A, CD218a, EC:3.2.2.6, IL 18R 1

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

APC-FcA98150 targets IL-18R1/CD218a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 18 receptor 1
• Calculated Molecular Weight: 541 aa, 62 kDa
• GenBank Accession Number: BC093975
• Gene Symbol: IL18R1
• Gene ID (NCBI): 8809
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

IL-18R1 (CD218a) is a component of the interleukin-18 receptor (IL-18R) complex, which is critical for the signaling of IL-18, a pro-inflammatory cytokine. This receptor complex, consisting of IL-18R1 and the IL-18 receptor accessory protein (IL-18RAP), belongs to the immunoglobulin-like family of interleukin-1 receptors (PMID: 35461924). IL-18R1 plays a significant role in inducing the production of interferon-gamma (IFN-γ) and other inflammatory cytokines, contributing to innate and adaptive immune responses (PMID: 2550053). It is also suggested to play a role in hypertension and chronic kidney disease, where the IL-18/IL-18R1 signaling axis may have diagnostic and therapeutic potential (PMID: 35461924).