IL-1F10 Monoclonal antibody, PBS Only

• 

  WB analysis of Recombinant protein using 60319-1-Ig (same clone as 60319-1-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60319-1-Ig (IL1F10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60319-1-PBS in a different storage buffer formulation.

• 

  FC experiment of U-937 using 60319-1-Ig (same clone as 60319-1-PBS)

  1X10^6 U-937 cells were intracellularly stained with 0.4 ug Anti-Human IL-1F10 (60319-1-Ig, Clone:5B2F5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60319-1-PBS in a different storage buffer formulation.

IL-1F10 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 60319-1-PBS

Clone No.5B2F5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

IL1F10, Family of interleukin 1-theta, FIL1 theta, FIL1T, FKSG75

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Product Information

60319-1-PBS targets IL-1F10 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17250

  Product name: Recombinant human IL-1F10 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-152 aa of BC103966

  Sequence: MCSLPMARYYIIKYADQKALYTRDGQLLVGDPVADNCCAEKICTLPNRGLDRTKVPIFLGIQGGSRCLACVETEEGPSLQLEDVNIEELYKGGEEATRFTFFQSSSGSAFRLEAAAWPGWFLCGPAEPQQPVQLTKESEPSARTKFYFEQSW

• Full Name: interleukin 1 family, member 10 (theta)
• Calculated Molecular Weight: 152 aa, 17 kDa
• GenBank Accession Number: BC103966
• Gene Symbol: IL1F10
• Gene ID (NCBI): 84639
• RRID: AB_2881430
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8WWZ1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IL1F10 is a member of the interleukin 1 cytokine family. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses.