FcZero-rAb® CoraLite® Plus 488 Anti-Human IL-2 Rabbit Recombinant Antibody

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• FcZero-rAb® CoraLite® Plus 488 Anti-Human IL-2 Rabbit Recombinant Antibody (CL488-FcA98053)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

IL-2 FcZero-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL488-FcA98053 Copied!

Clone No. 240416E3

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

IL2, IL 2, interleukin 2, Interleukin-2, TCGF

• 

  FC experiment of human PBMCs using CL488-FcA98053

  1x10^6 untreated or PMA and Iono treated human PBMCs were intracellularly stained with FcZero-rAb® CoraLite® Plus 750 Anti-Human CD3 and 5 ul CoraLite® Plus 488 Anti-Human IL-2 Rabbit RecAb (CL488-FcA98053, Clone: 240416E3). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using CL488-FcA98053

  1x10^6 PMA and Iono treated human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 488 Anti-Human IL-2 Rabbit RecAb (CL488-FcA98053, Clone: 240416E3) (red) or FcZero-rAb® CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-FcA98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 488 Anti-Human IL-2 Rabbit RecAb (CL488-FcA98053, Clone: 240416E3) (black, dashed). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: PMA and Iono treated human PBMCs

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-FcA98053 targets IL-2 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0828

  Product name: Recombinant Human IL-2 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 21-153 aa of BC070338

  Sequence: APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT

• Full Name: interleukin 2
• Calculated Molecular Weight: 153 aa, 18 kDa
• GenBank Accession Number: BC070338
• Gene Symbol: IL2
• Gene ID (NCBI): 3558
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P60568
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).