Anti-Mouse IL-21 Rabbit Recombinant Antibody

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  FC experiment of C57BL/6 Th17-polarized splenocytes using 98015-1-RR

  1x10^6 untreated or PMA, Ionomycin, and Brefeldin A treated C57BL/6 Th17-polarized splenocytes were intracellularly stained with APC Anti-Mouse CD4 (GK1.5) and 0.25 ug Anti-Mouse IL-21 Rabbit Recombinant Antibody (98015-1-RR, Clone: 230267G7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L).Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98015-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98015-1-RR against Mouse IL-21 were performed. The affinity constant is 1.29 nM.

IL-21 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98015-1-RR

Clone No.230267G7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Il21, interleukin 21, 230267G7

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Tested Applications

• Positive FC (Intra) detected in: PMA, Ionomycin and Brefeldin A treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98015-1-RR targets IL-21 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 21
• Calculated Molecular Weight: 17 aa
• GenBank Accession Number: NM_001291041
• Gene Symbol: IL-21
• Gene ID (NCBI): 60505
• RRID: AB_3672158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9ES17
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Interleukin 21 (IL-21) is a cytokine produced predominantly by cluster of differentiation 4 (CD4+) T-cells and natural killer T-cells. IL-21 is preliminarily expressed in activated cluster of differentiation 4 (CD4+) T-cells and natural killer (NK) T-cells. IL-21 receptor (IL-21R) is a class I cytokine receptor, which requires dimerization with the indispensable common gamma chain (γc) subunit to bind IL-21. IL-21 plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. IL-21 mediates apoptosis in B-CLL cells through up-regulation of the BIM (BH3 family member) and enhances both direct and antibody-dependent cellular cytotoxicity in these cells.