Anti-Human IL-3RB/CD131 Rabbit Recombinant Antibody

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  FC experiment of Transfected HEK-293T using 98336-2-RR

  1x10^6 HEK-293T cells (left) or IL-3RB/CD131-transfected HEK-293T cells (right) were surface stained with 0.25 ug Anti-Human IL-3 R Beta/CD131 Rabbit RecAb (98336-2-RR, Clone:241823B12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of Transfected HEK-293T using 98336-2-RR

  1x10^6 HEK-293T cells(left) or IL-3RB/CD131-transfected HEK-293T cells(right) were surface stained with 0.25 ug Anti-Human IL-3 R Beta/CD131 Rabbit RecAb (98336-2-RR, Clone:241823B12)(red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

IL-3RB/CD131 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98336-2-RR

Clone No.241823B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CSF2RB, CD131, CDw131, GM-CSF/IL-3/IL-5 receptor common beta subunit, IL3RB

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Tested Applications

• Positive FC detected in: Transfected HEK-293T cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98336-2-RR targets IL-3RB/CD131 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IL-3RB/CD131 fusion protein Eg1662
• Full Name: colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
• Calculated Molecular Weight: 97kDa
• GenBank Accession Number: NM_000395.3
• Gene Symbol: CSF2RB
• Gene ID (NCBI): 1439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P32927-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

IL-3RB/CD131, also known as CSF2RB, is the common subunit of the type I cytokine receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3 and IL-5. CD131 is expressed on various immune cells, including hematopoietic progenitor cells, granulocytes, monocytes, macrophages, eosinophils, and mast cells, which plays a crucial role in mediating the effects of cytokines (PMID: 18412971). Tregs of healthy donors constitutively transcribe CSF2RB, but protein expression was much higher after TCR stimulation (PMID: 36532080, 3557440).