Anti-Human IL-3RB/CD131 Rabbit Recombinant Antibody

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• Anti-Human IL-3RB/CD131 Rabbit Recombinant Antibody (98336-2-RR)

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  FC experiment of human peripheral blood leukocytes using 98336-2-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human IL-3RB/CD131 Rabbit RecAb (98336-2-RR, Clone: 241823B12) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98336-2-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human IL-3RB/CD131 Rabbit RecAb (98336-2-RR, Clone: 241823B12) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of Transfected HEK-293T using 98336-2-RR

  1x10^6 HEK-293T cells (left) or IL-3RB/CD131-transfected HEK-293T cells (right) were surface stained with 0.25 ug Anti-Human IL-3 R Beta/CD131 Rabbit RecAb (98336-2-RR, Clone:241823B12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of Transfected HEK-293T using 98336-2-RR

  1x10^6 HEK-293T cells(left) or IL-3RB/CD131-transfected HEK-293T cells(right) were surface stained with 0.25 ug Anti-Human IL-3 R Beta/CD131 Rabbit RecAb (98336-2-RR, Clone:241823B12)(red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

IL-3RB/CD131 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98336-2-RR

Clone No.241823B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CSF2RB, CD131, CDw131, GM-CSF/IL-3/IL-5 receptor common beta subunit, IL3RB

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes, Transfected HEK-293T cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98336-2-RR targets IL-3RB/CD131 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1662

  Product name: Recombinant Human IL-3RB/CD131 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 17-443 aa of NM_000395.3

  Sequence: WERSLAGAEETIPLQTLRCYNDYTSHITCRWADTQDAQRLVNVTLIRRVNEDLLEPVSCDLSDDMPWSACPHPRCVPRRCVIPCQSFVVTDVDYFSFQPDRPLGTRLTVTLTQHVQPPEPRDLQISTDQDHFLLTWSVALGSPQSHWLSPGDLEFEVVYKRLQDSWEDAAILLSNTSQATLGPEHLMPSSTYVARVRTRLAPGSRLSGRPSKWSPEVCWDSQPGDEAQPQNLECFFDGAAVLSCSWEVRKEVASSVSFGLFYKPSPDAGEEECSPVLREGLGSLHTRHHCQIPVPDPATHGQYIVSVQPRRAEKHIKSSVNIQMAPPSLNVTKDGDSYSLRWETMKMRYEHIDHTFEIQYRKDTATWKDSKTETLQNAHSMALPALEPSTRYWARVRVRTSRTGYNGIWSEWSEARSWDTESVLPMW

• Full Name: colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
• Calculated Molecular Weight: 97kDa
• GenBank Accession Number: NM_000395.3
• Gene Symbol: CSF2RB
• Gene ID (NCBI): 1439
• RRID: AB_3745986
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P32927-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

IL-3RB/CD131, also known as CSF2RB, is the common subunit of the type I cytokine receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3 and IL-5. CD131 is expressed on various immune cells, including hematopoietic progenitor cells, granulocytes, monocytes, macrophages, eosinophils, and mast cells, which plays a crucial role in mediating the effects of cytokines (PMID: 18412971). Tregs of healthy donors constitutively transcribe CSF2RB, but protein expression was much higher after TCR stimulation (PMID: 36532080, 3557440).