Anti-Rat IL-4 Rabbit Recombinant Antibody, PBS Only

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  FC experiment of Transfected HEK-293T using 98255-1-RR (same clone as 98255-1-PBS)

  1x10^6 HEK-293T cells or IL-4 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat IL-4 Rabbit Recombinant Antibody (98255-1-RR, Clone:241249H7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98255-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Transfected HEK-293T using 98255-1-RR (same clone as 98255-1-PBS)

  1x10^6 HEK-293T cells (left) or IL-4 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat IL-4 Rabbit Recombinant Antibody (98255-1-RR, Clone:241249H7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98255-1-PBS in a different storage buffer formulation.

IL-4 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98255-1-PBS

Clone No.241249H7

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC (Intra)

Synonyms

Il4, BSF-1, B-cell stimulatory factor 1, B-cell IGG differentiation factor, B-cell growth factor 1

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Product Information

98255-1-PBS targets IL-4 in FC (Intra) applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 4
• Calculated Molecular Weight: 16kDa
• GenBank Accession Number: NM_201270.1
• Gene Symbol: IL-4
• Gene ID (NCBI): 287287
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P20096
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IL-4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.