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Anti-Rat IL-4 Rabbit Recombinant Antibody (98255-1-RR)

Anti-Rat IL-4 Rabbit Recombinant Antibody

IL-4 antibodies are versatile tools for investigating IL-4’s roles in immunity, allergy, autoimmunity, and cancer, supporting a wide range of molecular, cellular, and functional assays. Proteintech has an extensive validated IL-4 antibodies for FC (Intra).

Cat No. 98255-1-RR

Clone No. 241249H7

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Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

FC (Intra)

Synonyms

241249H7, B-cell growth factor 1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BSF-1

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 647 CoraLite®594

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FC experiment of Transfected HEK-293T using 98255-1-RR
1x10^6 HEK-293T cells or IL-4 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat IL-4 Rabbit Recombinant Antibody (98255-1-RR, Clone:241249H7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of Transfected HEK-293T using 98255-1-RR
1x10^6 HEK-293T cells (left) or IL-4 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat IL-4 Rabbit Recombinant Antibody (98255-1-RR, Clone:241249H7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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1x10^6 HEK-293T cells (left) or IL-4 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat IL-4 Rabbit Recombinant Antibody (98255-1-RR, Clone:241249H7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

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Overview

Tested Applications
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MSDS SDS

Tested Applications

Positive FC (Intra) detected in

Transfected HEK-293T cells

Recommended dilution

Application	Dilution
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98255-1-RR targets IL-4 in FC (Intra) applications and shows reactivity with rat samples.

Tested Reactivity	rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg1113 Product name: Recombinant Rat IL-4 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 25-147 aa of NM_201270.1 Sequence: CNDSPLREIINTLNQVTEKGTPCTEMFVPDVLTATRNTTENELICRASRVLRKFYFPRDVPPCLKNKSGVLGELRKLCRGVSGLNSLRSCTVNESTLTTLKDFLESLKSILRGKYLQSCTSMS Predict reactive species
Full Name	interleukin 4
Calculated Molecular Weight	16kDa
GenBank Accession Number	NM_201270.1
Gene Symbol	IL-4
Gene ID (NCBI)	287287
RRID	AB_3745922
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P20096
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

IL-4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.