Anti-Mouse IL-5 Rabbit Recombinant Antibody

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  FC experiment of C57BL/6 Th2-polarized splenocytes using 98352-1-RR

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated C57BL/6 Th2-polarized splenocytes were intracellularly stained with 0.125 ug Anti-Mouse IL5 Rabbit RecAb (98352-1-RR, Clone:242075A7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98352-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98352-1-RR against Mouse IL-5 were performed. The affinity constant is 5.79 nM.

IL-5 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98352-1-RR

Clone No.242075A7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Il5, Il 5, interleukin 5, interleukin-5

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Tested Applications

• Positive FC (Intra) detected in: C57BL/6 Th2-polarized splenocytes

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98352-1-RR targets IL-5 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 5
• Calculated Molecular Weight: 15 aa
• GenBank Accession Number: NM_010558
• Gene Symbol: Il5
• Gene ID (NCBI): 16191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04401
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Interleukin-5 (IL-5) is a critical cytokine that regulates the growth, activation, and survival of eosinophils. IL-5 is produced by both hematopoietic and non-hematopoietic cells including T cells, granulocytes, and natural helper cells. IL-5 exerts its effects for proliferation and differentiation via receptors that comprise an IL-5-specific α and common β-subunit. Overexpression of IL-5 in vivo significantly increases eosinophils and B cells in number, while mice lacking a functional gene for IL-5 or IL-5 receptor display a number of developmental and functional impairments in B cells and eosinophil lineages.