Anti-Mouse IL-6 Rabbit Recombinant Antibody

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  FC experiment of RAW 264.7 using 98089-1-RR

  1x10^6 LPS treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL-6 Rabbit Recombinant Antibody (98089-1-RR, Clone:240701E8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L),or 1x10^6 untreated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL-6 Rabbit Recombinant Antibody (98089-1-RR, Clone:240701E8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of RAW 264.7 using 98089-1-RR

  1x10^6 LPS treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL-6 Rabbit Recombinant Antibody (98089-1-RR, Clone:240701E8) (red), or Isotype Control (blue), or 1x10^6 LPS treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL-6 Rabbit Recombinant Antibody (98089-1-RR, Clone:240701E8) (green)and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98089-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98089-1-RR against Mouse IL-6 were performed. The affinity constant is 0.795 nM.

IL-6 antibodies are used for both research and therapeutic purposes to detect, quantify, and block the activity of interleukin-6 (IL-6), a cytokine central to immune regulation, inflammation, hematopoiesis, and cancer biology. Proteintech has an extensive range of IL-6 antibodies validated for WB, IHC, IF/ICC, IP, Neutralization, ELISA and more.

Cat No. 98089-1-RR

Clone No.240701E8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Il6, interleukin-6, interleukin 6, Il 6, 240701E8

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Tested Applications

• Positive FC (Intra) detected in: LPS treated RAW 264.7 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98089-1-RR targets IL-6 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: interleukin 6
• Calculated Molecular Weight: 24 kDa
• GenBank Accession Number: NM_031168
• Gene Symbol: Il6
• Gene ID (NCBI): 16193
• RRID: AB_3672236
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P08505
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.