CoraLite® Plus 488 Anti-Human IL-7 Rabbit Recombinant Antibody

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• CoraLite® Plus 488 Anti-Human IL-7 Rabbit Recombinant Antibody (CL488-98206-10)

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  FC experiment of HUVEC using CL488-98206-10

  1x10^6 HUVEC cells were intracellularly stained with 5 ul CoraLite® Plus 488 Anti-Human IL-7 Rabbit RecAb (CL488-98206-10, Clone:252658G1) (red) or CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

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  FC experiment of Jurkat using CL488-98206-10

  1x10^6 Jurkat cells were intracellularly stained with 5 ul CoraLite® Plus 488 Anti-Human IL-7 Rabbit RecAb (CL488-98206-10, Clone:252658G1) (red) or CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

IL-7 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL488-98206-10

Clone No.252658G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

IL7, IL 7, interleukin 7, Interleukin-7

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Tested Applications

• Positive FC (Intra) detected in: HUVEC cells, Jurkat cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul / test per 10^6 cells in a 100 µl suspension or 5 ul / test per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-98206-10 targets IL-7 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo:

  Product name: HumanKine® recombinant human IL-7 protein

  Source: -derived,

  Tag:

  Domain: of

  Sequence:

• Full Name: interleukin 7
• Gene Symbol: IL-7
• Gene ID (NCBI): 3574
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13232
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin-7 (IL-7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL-7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL-7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration. (PMID: 29663382; 29655570; 29449560)