Mouse IL-1 alpha Recombinant antibody, PBS Only (Capture)

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  WB analysis of RAW 264.7 using 83644-1-RR (same clone as 83644-1-PBS)

  Untreated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83644-1-RR (IL-1alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83644-1-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 83644-1-RR (same clone as 83644-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed LPS treated RAW 264.7 cells using IL-1alpha antibody (83644-1-RR, Clone: 240431B5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83644-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00634-2

  Cytometric bead array standard curve of MP00634-2, MOUSE IL-1 alpha Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83644-1-PBS. Detection antibody: 83644-2-PBS. Standard: Eg0263. Range: 0.78-50 ng/mL.

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  Affinity and Kinetic Characterization of 83644-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83644-1-RR against Mouse IL-1alpha were performed. The affinity constant is 1.15 nM.

IL-1 alpha Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 83644-1-PBS

Clone No.240431B5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

IL1-alpha, Il1a, IL-1,IL1, IL1 alpha, Il 1a

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Product Information

83644-1-PBS targets IL-1 alpha as part of a matched antibody pair:

MP00634-2: 83644-1-PBS capture and 83644-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 1 alpha
• Calculated Molecular Weight: 31kd
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: NM_010554
• Gene Symbol: Il1a
• Gene ID (NCBI): 16175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01582
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-1α (IL-1 Alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 Alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 Alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1 Alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 Alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL-1 Alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages.