IL-12B Polyclonal antibody

IL-12B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17645-1-AP

Synonyms

CLMF, CLMF p40, CLMF2, IL 12 subunit p40, IL 12B, IL12B, IL-12B, Interleukin 12 subunit beta, NKSF, NKSF2

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Validation Data Gallery

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  WB analysis of Recombinant protein using 17645-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 17645-1-AP (IL-12B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Recombinant protein, Recombinant protein protein

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of 17645-1-AP is IL-12B Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-12B fusion protein Ag11837
• Full Name: interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
• Calculated molecular weight: 328 aa, 37 kDa
• Observed molecular weight: 39 kDa
• GenBank accession number: BC067498
• Gene symbol: IL12B
• Gene ID (NCBI): 3593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 is a 75 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40) that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells. IL-12B has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of IL-12B was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of IL-12B has been reported to be associated with the severity of atopic and non-atopic asthma in children.

Publications

Species	Application	Title
human	IF	Int J Pharm; Dual-targeting Tumor Cells and Tumor Associated Macrophages with Lipid Coated Calcium Zoledronate for Enhanced Lung Cancer Chemoimmunotherapy.; Authors - Xinlong Zang