IL-12B Polyclonal antibody
IL-12B Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 17645-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Recombinant protein, Recombinant protein protein |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Published Applications
IF | See 1 publications below |
Product Information
The immunogen of 17645-1-AP is IL-12B Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IL-12B fusion protein Ag11837 |
Full Name | interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) |
Calculated molecular weight | 328 aa, 37 kDa |
Observed molecular weight | 39 kDa |
GenBank accession number | BC067498 |
Gene symbol | IL12B |
Gene ID (NCBI) | 3593 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 is a 75 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40) that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells. IL-12B has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of IL-12B was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of IL-12B has been reported to be associated with the severity of atopic and non-atopic asthma in children.
Protocols
Product Specific Protocols | |
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WB protocol for IL-12B antibody 17645-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Int J Pharm Dual-targeting Tumor Cells and Tumor Associated Macrophages with Lipid Coated Calcium Zoledronate for Enhanced Lung Cancer Chemoimmunotherapy. |