Tested Applications
| Positive WB detected in | human brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60245-1-Ig targets IL-17F in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16310 Product name: Recombinant human IL-17F protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-163 aa of BC070124 Sequence: MTVKTLHGPAMVKYLLLSILGLAFLSEAAARKIPKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHRVQ Predict reactive species |
| Full Name | interleukin 17F |
| Calculated Molecular Weight | 163 aa, 18 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC070124 |
| Gene Symbol | IL-17F |
| Gene ID (NCBI) | 112744 |
| RRID | AB_2881367 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96PD4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The interleukin 17 (IL-17) family of cytokines contains 6 structurally related cytokines, IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17 family plays crucial roles in host defense against microbial organisms and in the development of inflammatory diseases. IL-17A is a pro-inflammatory cytokine that also has the capacity to promote angiogenesis and osteoclastogenesis. IL-17F shares the highest homology with IL-17A and signals via a receptor composed by the IL-17RA and IL-17RC subunits. IL-17A and IL-17F can form IL-17A/A or IL-17F/F homodimers, IL-17A/F heterodimers are also formed. IL-17A and IL-17F, produced by the Th17 CD4(+) T cell lineage, have been linked to a variety of inflammatory and autoimmune conditions. IL-17F levels are elevated in sera and lesional psoriatic skin compared to non-lesional tissue. IL-17F also has been implicated in the development of neutrophilic airway inflammation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-17F antibody 60245-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IL-17F Monoclonal antibody (6H5F10), catalog number 60245-1-Ig, has 1 total citation. The cited publication appeared in the Journal of Cutaneous Pathology (J Cutan Pathol), a peer-reviewed journal in dermatopathology. The research field is dermatology and inflammatory skin disease, specifically examining pyoderma gangrenosum associated with iatrogenic interleukin 17A blockade. The antibody was used in IHC applications on human samples.
| Species | Application | Title |
|---|---|---|
J Cutan Pathol Pyoderma Gangrenosum Associated With Iatrogenic Interleukin 17A Blockade: A Report of Two Cases and a Review of the Literature |
