|Positive WB detected in||HeLa cells, A431 cells, HaCaT cells, DU 145 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10663-1-AP targets IL-18 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IL-18 fusion protein Ag1063|
|Calculated molecular weight||193 aa, 22 kDa|
|Observed molecular weight||22 kDa|
|GenBank accession number||BC007461|
|Gene ID (NCBI)||3606|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form. This antibody 10663-1-AP is raised against the full length IL18 precursor, and it recognizes both the pre-IL18 and mature IL18 theoretically.
|Product Specific Protocols|
|WB protocol for IL-18 antibody 10663-1-AP||Download protocol|
|IHC protocol for IL-18 antibody 10663-1-AP||Download protocol|
|IF protocol for IL-18 antibody 10663-1-AP||Download protocol|
|IP protocol for IL-18 antibody 10663-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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