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IL-18 Polyclonal antibody

IL-18 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10663-1-AP

Synonyms

Iboctadekin, IFN gamma inducing factor, IGIF, IL 1 gamma, IL 18, IL 1g, IL18, IL-18, IL1F4, Interleukin 1 gamma, Interleukin 18, Mature IL18, pre IL18, Pre-IL18



Tested Applications

Positive WB detected inHeLa cells, A431 cells, HaCaT cells, DU 145 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10663-1-AP targets IL-18 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-18 fusion protein Ag1063
Full Name IL-18
Calculated molecular weight 193 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC007461
Gene symbol IL18
Gene ID (NCBI) 3606
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form. This antibody 10663-1-AP is raised against the full length IL18 precursor, and it recognizes both the pre-IL18 and mature IL18 theoretically.

Protocols

Product Specific Protocols
WB protocol for IL-18 antibody 10663-1-APDownload protocol
IHC protocol for IL-18 antibody 10663-1-APDownload protocol
IF protocol for IL-18 antibody 10663-1-APDownload protocol
IP protocol for IL-18 antibody 10663-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mediators Inflamm

Astragaloside IV Suppresses High Glucose-Induced NLRP3 Inflammasome Activation by Inhibiting TLR4/NF-κB and CaSR.

Authors - Bin Leng
humanWB

Plant Foods Hum Nutr

Sorghum Phenolics Inhibits Inflammasomes in Lipopolysaccharide (LPS)-Primed and Adenosine Triphosphate (ATP)-Activated Macrophages.

Authors - Vermont P Dia
humanIHC

Front Oncol

Antitumor Effects of Berberine on Gliomas via Inactivation of Caspase-1-Mediated IL-1β and IL-18 Release.

Authors - Lei Tong
ratWB

Cells

Parkin-Dependent Mitophagy is Required for the Inhibition of ATF4 on NLRP3 Inflammasome Activation in Cerebral Ischemia-Reperfusion Injury in Rats.

Authors - Qi He
humanWB

Coron Artery Dis

NLRP3 inflammasome in peripheral blood monocytes of acute coronary syndrome patients and its relationship with statins.

Authors - Afrasyab Altaf
ratWB

Biomed Pharmacother

Autophagy inhibition attenuates the induction of anti-inflammatory effect of catalpol in liver fibrosis.

Authors - Zhaoguo Liu