Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, DU 145 cells, HaCaT cell, HaCaT cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10663-1-AP targets IL-18 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, chicken, zebrafish, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1063 Product name: Recombinant human Pre IL-18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-193 aa of BC007461 Sequence: MAAEPVEDNCINFVAMKFIDNTLYFIAEDDENLESDYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED Predict reactive species |
| Full Name | IL-18 |
| Calculated Molecular Weight | 193 aa, 22 kDa |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | BC007461 |
| Gene Symbol | IL-18 |
| Gene ID (NCBI) | 3606 |
| RRID | AB_2123636 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14116 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form. This antibody 10663-1-AP is raised against the full length IL18 precursor, and it recognizes both the pre-IL18 and mature IL18 theoretically.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IL-18 antibody 10663-1-AP | Download protocol |
| IF protocol for IL-18 antibody 10663-1-AP | Download protocol |
| IP protocol for IL-18 antibody 10663-1-AP | Download protocol |
| WB protocol for IL-18 antibody 10663-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IL-18 Polyclonal antibody (Cat# 10663-1-AP) has 527 total citations published across 80+ journals, including Cell, Nature Communications, Molecular Cancer, Cell Death & Disease, Redox Biology, Journal of Neuroinflammation, and Phytomedicine. Key research fields include inflammasome-mediated pyroptosis (NLRP3/AIM2/NLRC4), neuroinflammation, cardiovascular disease, oncology, liver and kidney injury, metabolic disorders, and natural product pharmacology. Primary applications are WB, IHC, IF, IP, ELISA, and flow cytometry.
| Species | Application | Title |
|---|---|---|
Mol Cancer Anti irradiation nanoparticles shelter immune organ from radio-damage via preventing the IKK/IκB/NF-κB activation | ||
Cell Mol Immunol Pik3c3 deficiency in myeloid cells imparts partial resistance to experimental autoimmune encephalomyelitis associated with reduced IL-1β production.
| ||
Nat Commun IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence. | ||
Nat Commun Mechanics-activated fibroblasts promote pulmonary group 2 innate lymphoid cell plasticity propelling silicosis progression |
Reviews
What customers say
The single review for this antibody (rated 4/5) highlights strong labeling performance in immunohistochemistry on frontal tissue. The reviewer noted that achieving optimal results required multiple rounds of titration due to elevated background at lower dilutions, ultimately settling on a 1:5000 working dilution for clean staining. Overall, the antibody was well received for its specific labeling once the correct dilution was identified.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jillian (Verified Customer) (05-27-2025) | Great antibody, labels very well. We had to titrate multiple times to get to the working value because it had a high background until we reached 1:5000 dilution
|
