IL-1 Beta Monoclonal antibody
IL-1 Beta Monoclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (2)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1C6F12
Cat no : 60136-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Recombinant protein |
Positive IP detected in | RAW 264.7 cells |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 10 publications below |
ELISA | See 1 publications below |
Product Information
60136-1-Ig targets IL-1 Beta in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | IL-1 Beta fusion protein Ag10295 |
Full Name | interleukin 1, beta |
Calculated molecular weight | 269 aa, 31 kDa |
GenBank accession number | BC008678 |
Gene symbol | IL1B |
Gene ID (NCBI) | 3553 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15 to 18 kDa protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.
Protocols
Product Specific Protocols | |
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WB protocol for IL-1 Beta antibody 60136-1-Ig | Download protocol |
IHC protocol for IL-1 Beta antibody 60136-1-Ig | Download protocol |
IP protocol for IL-1 Beta antibody 60136-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Immunol RNA viruses promote activation of the NLRP3 inflammasome through a RIP1-RIP3-DRP1 signaling pathway. | ||
Nat Cell Biol Myeloid PTEN promotes chemotherapy-induced NLRP3-inflammasome activation and antitumour immunity. | ||
Nat Commun CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation. | ||
Oncotarget Loss of AIM2 expression promotes hepatocarcinoma progression through activation of mTOR-S6K1 pathway. | ||
J Neuroinflammation Protection of ischemic post conditioning against transient focal ischemia-induced brain damage is associated with inhibition of neuroinflammation via modulation of TLR2 and TLR4 pathways. |