IL-1 Beta Monoclonal antibody

IL-1 Beta Monoclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (2)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

11

CloneNo.

1C6F12

Cat no : 60136-1-Ig

Synonyms

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Validation Data Gallery

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  WB analysis of Recombinant protein using 60136-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 60136-1-Ig (IL1 beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of RAW 264.7 using 60136-1-Ig

  IP Result of anti-IL1 beta (IP:60136-1-Ig, 5ug; Detection:60136-1-Ig 1:600) with RAW 264.7 cells lysate 4000ug.

• 

  IHC staining of human skin cancer using 60136-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60136-1-Ig (IL1 beta antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 60136-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60136-1-Ig (IL1 beta antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Recombinant protein
• Positive IP detected in: RAW 264.7 cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 10 publications below
• ELISA: See 1 publications below

Product Information

60136-1-Ig targets IL-1 Beta in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-1 Beta fusion protein Ag10295
• Full Name: interleukin 1, beta
• Calculated molecular weight: 269 aa, 31 kDa
• GenBank accession number: BC008678
• Gene symbol: IL1B
• Gene ID (NCBI): 3553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15 to 18 kDa protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets. The expression level of IL-1B is very low.

Publications

Species	Application	Title
human	WB	Nat Immunol; RNA viruses promote activation of the NLRP3 inflammasome through a RIP1-RIP3-DRP1 signaling pathway.; Authors - Xiaqiong Wang
human,mouse	WB	Nat Cell Biol; Myeloid PTEN promotes chemotherapy-induced NLRP3-inflammasome activation and antitumour immunity.; Authors - Yi Huang
human,mouse	WB	Nat Commun; CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation.; Authors - Tiantian Tang
human	WB	EMBO Mol Med; Tranilast directly targets NLRP3 to treat inflammasome-driven diseases.; Authors - Yi Huang
human	WB	Oncotarget; Loss of AIM2 expression promotes hepatocarcinoma progression through activation of mTOR-S6K1 pathway.; Authors - Xiaomin Ma
rat	WB	J Neuroinflammation; Protection of ischemic post conditioning against transient focal ischemia-induced brain damage is associated with inhibition of neuroinflammation via modulation of TLR2 and TLR4 pathways.; Authors - Ying Wang