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IL-1R2 Monoclonal antibody

IL-1R2 Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3H4H4

Cat no : 60262-1-Ig

Synonyms

CD121b, CDw121b, IL 1R 2, IL 1R beta, IL 1RT 2, IL 1RT2, IL1 Receptor 2, IL-1 Receptor 2, IL1 Receptor II, IL-1 Receptor II, IL1R2, IL-1R2, IL1RB, IL-1RII, Interleukin 1 receptor beta, Interleukin 1 receptor type 2, Interleukin 1 receptor type II

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IL-1R2 Monoclonal antibody (60262-1-Ig)

    WB analysis of HeLa using 60262-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60262-1-Ig (IL1R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IL-1R2 Monoclonal antibody (60262-1-Ig)

    IF Staining of HeLa using 60262-1-Ig

    Immunofluorescent analysis of HeLa cells using 60262-1-Ig (IL1R2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 60262-1-Ig is IL-1R2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IL-1R2 fusion protein Ag4495
Full Name interleukin 1 receptor, type II
Calculated Molecular Weight 398 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC039031
Gene Symbol IL1R2
Gene ID (NCBI) 7850
RRIDAB_2881383
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1 receptor type 2 (IL1R2, also known as CD121b) is a cytokine receptor that belongs to the interleukin 1 receptor family. It exists in both a membrane bound and soluble form. IL1R2 binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 serves as a negative regulator of IL-1 signaling, thereby suppressing inflammatory response.

Protocols

Product Specific Protocols
WB protocol for IL-1R2 antibody 60262-1-IgDownload protocol
IF protocol for IL-1R2 antibody 60262-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mater Today Bio

Intracellular hydrogelation of macrophage conjugated probiotics for hitchhiking delivery and combined treatment of colitis

Authors - Jingzhe Wang
View Article
mouseIF,WB

Biochem Pharmacol

Oridonin employs interleukin 1 receptor type 2 to treat noise-induced hearing loss by blocking inner ear inflammation

Authors - Meng-Bing Chen
View Article