Tested Applications
| Positive WB detected in | Recombinant protein, Ag21436 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66164-1-Ig targets IL-27A p28 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21436 Product name: Recombinant human IL-27 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 19-243 aa of BC062422 Sequence: LLLVQAGVWGFPRPPGRPQLSLQELRREFTVSLHLARKLLAEVRGQAHRFAESHLPGVNLYLLPLGEQLPDVSLTFQAWRRLSDPERLCFISTTLQPFHALLGGLGTQGRWTNMERMQLWAMRLDLRDLQRHLRFQVLAAGFNLPEEEEEEEEEEEEERKGLLPGALGSALQGPAQVSWPQLLSTYRLLHSLELVLSRAVRELLLLSKAGHSVWPLGFPTLSPQP Predict reactive species |
| Full Name | interleukin 27 |
| Calculated Molecular Weight | 243 aa, 27 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC062422 |
| Gene Symbol | IL-27 |
| Gene ID (NCBI) | 246778 |
| RRID | AB_2881560 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8NEV9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IL-27, as a member of the IL-6/IL-12 family, is a heterodimeric cytokine composed of two subunits: p28 (IL-27a) and EBI3 (IL-27b). IL-27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL-27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL-10 producing type 1 regulatory T cells. IL-27 is a potentially promising cytokine for therapeutic approaches on various human diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-27A p28 antibody 66164-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IL-27A p28 Monoclonal antibody (1E1H5), catalog number 66164-1-Ig, has 2 total citations. These appear in iScience (2025), studying fibroblast remodeling in thyroid-associated ophthalmopathy using WB and IHC, and in Anticancer Research (2021), examining peritumoral lymphocyte structure in type II endometrial cancer using IHC. Both studies were conducted in human samples. The research fields span autoimmune ophthalmic disease and oncology.
| Species | Application | Title |
|---|---|---|
iScience Interleukin-27 is a multitarget regulator of fibroblast remodeling in thyroid-associated ophthalmopathy. | ||
Anticancer Res The Prognostic Significance of Peritumoral Lymphocytes' Band-like Structure in Type II Endometrial Cancer. |
