Filter: AllWBIF/ICCAffinity

WB analysis of CHO using 86548-3-RR (same clone as 86548-3-PBS)
Transfected with Human IL29 CHO cells were subjected to SDS PAGE followed by western blot with 86548-3-RR (IL29 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86548-3-PBS in a different storage buffer formulation.
IF Staining of Transfected CHO-K1 using 86548-3-RR (same clone as 86548-3-PBS)
Immunofluorescent analysis of (4% PFA) fixed Transfected CHO-K1 using IL-29 antibody (86548-3-RR, Clone: 250226F7 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 86548-3-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 86548-3-RR
Biolayer interferometry (BLl) kinetic assays of 86548-3-RR against Human IL-29 were performed. The affinity constant is 26.2 nM.

IL-29 Recombinant monoclonal antibody, PBS Only

Name: IL-29 Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 86548-3-PBS

IL-29 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86548-3-PBS

Clone No.250226F7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

IL29, Cytokine Zcyto21, IFN lambda 1, IFNL1, IFN-lambda-1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

86548-3-PBS targets IL-29 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg2843 Product name: Recombinant Human IL-29 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 20-200 aa of NM_172140.1 Sequence: GPVPTSKPTTTGKGCHIGRFKSLSPQELASFKKARDALEESLKLKNWSCSSPVFPGNWDLRLLQVRERPVALEAELALTLKVLEAAAGPALEDVLDQPLHTLHHILSQLQACIQPQPTAGPRPRGRLHHWLHRLQEAPKKESAGCLEASVTFNLFRLLTRDLKYVADGNLCLRTSTHPEST Predict reactive species
Full Name	interleukin 29 (interferon, lambda 1)
Calculated Molecular Weight	22 kDa
Observed Molecular Weight	22 kDa
GenBank Accession Number	NM_172140.1
Gene Symbol	IL-29
Gene ID (NCBI)	282618
RRID	AB_3744959
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8IU54
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

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