CD25/IL-2RA Recombinant antibody, PBS Only

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  WB analysis of MJ using 83896-1-RR (same clone as 83896-1-PBS)

  MJ cells were subjected to SDS PAGE followed by western blot with 83896-1-RR (IL2RA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83896-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83896-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83896-1-RR against Human CD25/IL2RA were performed. The affinity constant is 31.7 nM.

CD25/IL-2RA Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 83896-1-PBS

Clone No.240906A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

CD25, 240906A12, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha

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Product Information

83896-1-PBS targets CD25/IL-2RA in WB, Cytometric bead array, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD25/IL-2RA fusion protein Eg0408
• Full Name: interleukin 2 receptor, alpha
• Calculated Molecular Weight: 272 aa, 31 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC156627
• Gene Symbol: IL2RA
• Gene ID (NCBI): 3559
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01589
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present in activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+ Foxp3+ Treg cells (PMID: 22585674).