CoraLite® Plus 488-conjugated IL-33 Monoclonal antibody

IL-33 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A9C7

Cat no : CL488-66235

Synonyms

C9orf26, DKFZp586H0523, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-66235

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66235 (IL33 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

CL488-66235 targets IL-33 in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-33 fusion protein Ag21430
• Full Name: interleukin 33
• Calculated molecular weight: 30 kDa
• Observed molecular weight: 35 kDa
• GenBank accession number: BC047085
• Gene symbol: IL33
• Gene ID (NCBI): 90865
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-33 is an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.