Biotin Anti-Human IL3RA/CD123 Rabbit Recombinant Antibody

• 

  FC experiment of human monocyte-derived immature dendritic cells using Biotin-98107

  1x10^6 human monocyte-derived immature dendritic cells were surface stained with 0.25 ug Biotin Plus Anti-Human IL3RA/CD123 Rabbit Recombinant Antibody (Biotin-98107, Clone: 241268E3) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD45RO (UCHL1) (CL647-65150, Clone: UCHL1). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human monocyte-derived immature dendritic cells using Biotin-98107

  1x10^6 human monocyte-derived immature dendritic cells were surface stained with 0.25 ug Biotin Plus Anti-Human IL3RA/CD123 Rabbit Recombinant Antibody (Biotin-98107, Clone: 241268E3) (red) and CoraLite®488-conjugated streptavidin, or unstained (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

IL3RA/CD123 Recombinant Antibody for FC

Cat No. Biotin-98107

Clone No.241268E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra

---

Tested Applications

• Positive FC detected in: human monocyte-derived immature dendritic cells

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-98107 targets IL3RA/CD123 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: interleukin 3 receptor, alpha (low affinity)
• Calculated Molecular Weight: 378 aa, 43 kDa
• GenBank Accession Number: BC035407
• Gene Symbol: CD123
• Gene ID (NCBI): 3563
• ENSEMBL Gene ID: ENSG00000185291
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P26951
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin-3 receptor subunit alpha (IL3RA), also known as CD123, belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). IL3RA is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to IL3RA and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.