FcZero-rAb® Biotin Anti-Human IL3RA/CD123 Rabbit Recombinant Antibody

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• FcZero-rAb® Biotin Anti-Human IL3RA/CD123 Rabbit Recombinant Antibody (Biotin-FcA98107)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

IL3RA/CD123 FcZero-rAb^® Recombinant Antibody for FC, ELISA

Cat No. Biotin-FcA98107 Copied!

Clone No. 241268E3

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, ELISA

Synonyms

IL3RA, CD123, hIL 3Ra, IL 3 receptor subunit alpha, IL 3R alpha

• 

  FC experiment of human PBMCs using Biotin-FcA98107

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human IL3RA/CD123 Rabbit RecAb (Biotin-FcA98107, Clone: 241268E3) or FcZero-rAb® Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9), and Streptavidin-PE Conjugate (PE-PF00030). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD45RO (UCHL1) (CL647-65150, Clone: UCHL1). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using Biotin-FcA98107

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human IL3RA/CD123 Rabbit RecAb (Biotin-FcA98107, Clone: 241268E3) (red) or FcZero-rAb® Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98107 targets IL3RA/CD123 in FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: interleukin 3 receptor, alpha (low affinity)
• Calculated Molecular Weight: 378 aa, 43 kDa
• GenBank Accession Number: BC035407
• Gene Symbol: CD123
• Gene ID (NCBI): 3563
• ENSEMBL Gene ID: ENSG00000185291
• RRID: AB_3746922
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P26951
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Interleukin-3 receptor subunit alpha (IL3RA), also known as CD123, belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). IL3RA is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to IL3RA and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.