ILT-3/LILRB4 Polyclonal antibody

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• ILT-3/LILRB4 Polyclonal antibody (28422-1-AP)

• 

  WB analysis using 28422-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 28422-1-AP (ILT-3/LILRB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ILT-3/LILRB4 Polyclonal Antibody for WB, ELISA

Cat No. 28422-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Synonyms

LILRB4, B4, CD85 antigen-like family member K, CD85k, ILT3

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Tested Applications

• Positive WB detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Product Information

28422-1-AP targets ILT-3/LILRB4 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28928

  Product name: Recombinant human LILRB4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 52-176 aa of BC026309

  Sequence: TLEAREYRLDKEESPAPWDRQNPLEPKNKARFSIPSMTEDYAGRYRCYYRSPVGWSQPSDPLELVMTGAYSKPTLSALPSPLVTSGKSVTLLCQSRSPMDTFLLIKERAAHPLLHLRSEHGAQQH

• Full Name: leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4
• Calculated Molecular Weight: 448 aa, 49 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC026309
• Gene Symbol: LILRB4
• Gene ID (NCBI): 11006
• RRID: AB_3086050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NHJ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LILRB4 is an inhibitory receptor in the LILR family mainly expressed on normal and malignant human cells of myeloid origin. LILRB4 is activated and subsequently recruits adaptors to cytoplasmic immunoreceptor tyrosine inhibitory motifs to initiate different signaling cascades, thus playing an important role in physiological and pathological conditions, including autoimmune diseases, microbial infections, and cancers.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB,IF	Cancer Immunol Immunother; NLRP12/C1qA positive feedback in tumor-associated macrophages regulates immunosuppression through LILRB4/NF-κB pathway in lung adenocarcinoma; Authors - Jiaxin Yin ; KD Validated
	WB,IF	Hum Genomics; APM⁺ macrophages associated with plaque vulnerability via MIF-CD74 signaling: a multi-omics study.; Authors - Xiang Xu
human	WB	PLoS One; The combination of LILRB4-targeting NK cell engagers and cGAS-STING agonists enhances the anti-multiple myeloma immune activity of NK cells.; Authors - Meng Li