Anti-Human ILT-3/LILRB4 Rabbit Recombinant Antibody

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• Anti-Human ILT-3/LILRB4 Rabbit Recombinant Antibody (98486-3-RR)

ILT-3/LILRB4 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98486-3-RR

Clone No.250356B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

LILRB4, B4, CD85 antigen-like family member K, CD85k, ILT3

• 

  FC experiment of human PBMCs using 98486-3-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ILT-3/LILRB4 Rabbit RecAb (98486-3-RR, Clone: 250356B3) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L).Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using 98486-3-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human ILT-3/LILRB4 Rabbit RecAb (98486-3-RR, Clone: 250356B3) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98486-3-RR targets ILT-3/LILRB4 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1743

  Product name: Recombinant Human ILT-3/LILRB4 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 22-259 aa of AAH26309.1

  Sequence: QAGPLPKPTLWAEPGSVISWGNSVTIWCQGTLEAREYRLDKEESPAPWDRQNPLEPKNKARFSIPSMTEDYAGRYRCYYRSPVGWSQPSDPLELVMTGAYSKPTLSALPSPLVTSGKSVTLLCQSRSPMDTFLLIKERAAHPLLHLRSEHGAQQHQAEFPMSPVTSVHGGTYRCFSSHGFSHYLLSHPSDPLELIVSGSLEGPRPSPTRSVSTAAGPEDQPLMPTGSVPHSGLRRHWE

• Full Name: leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4
• Calculated Molecular Weight: 49kDa
• GenBank Accession Number: AAH26309.1
• Gene Symbol: LILRB4
• Gene ID (NCBI): 11006
• RRID: AB_3746116
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NHJ6
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

LILRB4 is an inhibitory receptor in the LILR family mainly expressed on normal and malignant human cells of myeloid origin. LILRB4 is activated and subsequently recruits adaptors to cytoplasmic immunoreceptor tyrosine inhibitory motifs to initiate different signaling cascades, thus playing an important role in physiological and pathological conditions, including autoimmune diseases, microbial infections, and cancers.