CoraLite® Plus 488-conjugated IMMT Monoclonal antibody

IMMT Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D2E8

Cat no : CL488-68226

Synonyms

DKFZp779P1653, HMP, IMMT, Mitofilin, P87, P87/89, P89, PIG4, PIG52

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Validation Data Gallery

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  IF Staining of HeLa using CL488-68226

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 IMMT antibody (CL488-68226, Clone: 2D2E8 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68226 targets IMMT in IF applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IMMT fusion protein Ag0102
• Full Name: inner membrane protein, mitochondrial (mitofilin)
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC002412
• Gene Symbol: IMMT
• Gene ID (NCBI): 10989
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.