INF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

INF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

32

Reviews (2)

Cat no : 20466-1-AP

Synonyms

Inverted formin-2, Inverted formin 2, HBEBP2-binding protein C, HBEBP2 binding protein C, C14orf173

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Validation Data Gallery

• 

  WB analysis using 20466-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20466-1-AP (INF2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20466-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20466-1-AP (INF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 20466-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20466-1-AP (INF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 20466-1-AP

  IP result of anti-INF2 (IP:20466-1-AP, 4ug; Detection:20466-1-AP 1:1500) with NIH/3T3 cells lysate 1505 ug.

• 

  IHC staining of human kidney using 20466-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20466-1-AP (INF2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 20466-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20466-1-AP (INF2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 20466-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using INF2 antibody (20466-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, NIH/3T3 cells, HeLa cells, PC-3 cells, K-562 cells
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 23 publications below
• IHC: See 3 publications below
• IF: See 18 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

20466-1-AP targets INF2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INF2 fusion protein Ag14302
• Full Name: inverted formin, FH2 and WH2 domain containing
• Calculated Molecular Weight: 1249 aa, 136 kDa
• Observed Molecular Weight: 180-200 kDa
• GenBank Accession Number: BC008756
• Gene Symbol: INF2
• Gene ID (NCBI): 64423
• RRID: AB_10694821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INF2 is a member of the formin family of actin-regulating proteins. INF2 is peripherally bound to the cytoplasmic face of the endoplasmic reticulum (ER). Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis. The predicted MW of INF2 is around 136 kDa, while higher molecular mass from 140-200 kDa has been reported in literature (26383224,20493814,19366733).

Publications

Species	Application	Title
mouse	IF	Cell; FMN2 Makes Perinuclear Actin to Protect Nuclei during Confined Migration and Promote Metastasis.; Authors - Colleen T Skau
mouse	IF	Nat Commun; GPCR-induced calcium transients trigger nuclear actin assembly for chromatin dynamics.; Authors - Ying Wang
human	IF	EMBO J; Mechanical strain stimulates COPII-dependent secretory trafficking via Rac1; Authors - Santosh Phuyal
mouse	IF	Proc Natl Acad Sci U S A; Inverted formin 2 in focal adhesions promotes dorsal stress fiber and fibrillar adhesion formation to drive extracellular matrix assembly.; Authors - Colleen T Skau
human	WB, IF	Proc Natl Acad Sci U S A; Mechanical stimulation induces formin-dependent assembly of a perinuclear actin rim.; Authors - Xiaowei Shao ; KD Validated
zebrafish	WB,IF	Curr Biol; Calcium Wave Promotes Cell Extrusion.; Authors - Yasuto Takeuchi ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (11-05-2023)

Excellent antibody. Detects a band at the correct MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HUVEC

INF2 Antibody Western Blot validation (1:1000 dilution) in HUVEC (Cat no:20466-1-AP)

FH

Chun (Verified Customer) (07-03-2019)

This antibody is good.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2,000
• Cell Tissue Type: HEK293

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