Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66910 targets INF2 in IF/ICC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17043 Product name: Recombinant human INF2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC008756 Sequence: MVRPKAQLVLAACESLLTSRQLPIFCQLILRIGNFLNYGSHTGDADGFKISTLLKLTETKSQQNRVTLLHHVLEEAEKSHPDLLQLPRDLEQPSQAAGINLEIIRSEASSNLKKLLETERKVSASVAEVQEQYTERLQASISAFRALDELFEAIEQKQRELADYLCEDAQQLSLEDTFSTMKAFRDLFLRALKENKDRKEQAAKAERRKQQLAEEEARRPRGEDGKPVRKGPGKQEEVCVIDALLADIRKGFQLRKTARGRGDTDGGSKAASMDPPRATEPVATSNPAGDPVGSTRCPASEPGLDATTASESRGWDLVDAVTPGPQPTLEQLEEGGPRPLERRSSWYVDA Predict reactive species |
| Full Name | inverted formin, FH2 and WH2 domain containing |
| Calculated Molecular Weight | 1249 aa, 136 kDa |
| Observed Molecular Weight | 150-170 kDa |
| GenBank Accession Number | BC008756 |
| Gene Symbol | INF2 |
| Gene ID (NCBI) | 64423 |
| RRID | AB_2919386 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q27J81 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
INF2 is a member of the formin family of actin-regulating proteins. INF2 is peripherally bound to the cytoplasmic face of the endoplasmic reticulum (ER). Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis. The predicted MW of INF2 is around 136 kDa, while higher molecular mass from 140-200 kDa has been reported in literature (26383224,20493814,19366733).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 INF2 antibody CL488-66910 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CL488-66910 is an INF2 Monoclonal antibody (clone 4A11A7), conjugated with CoraLite® Plus 488. It has 1 total citation, published in Kidney360 (2024). The cited study investigated AMPK-SP1-guided dynein expression as an energy-responsive mechanism and therapeutic target for diabetic nephropathy in mouse models, using IF application. The research field encompasses renal pathology, diabetic nephropathy, and cellular energy metabolism signaling.
| Species | Application | Title |
|---|---|---|
Kidney360 AMPK-SP1-Guided Dynein Expression Represents a New Energy-Responsive Mechanism and Therapeutic Target for Diabetic Nephropathy |
