Inhibin beta A-Specific Polyclonal antibody

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• Inhibin beta A-Specific Polyclonal antibody (17524-1-AP)

Inhibin beta A-Specific Polyclonal Antibody for WB, IP, ELISA

Cat No. 17524-1-AP Copied!

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, Inhibin β-A, inhibin, beta A

• 

  WB analysis of rat brain using 17524-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17524-1-AP (Inhibin beta A-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 17524-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17524-1-AP (Inhibin beta A-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 17524-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17524-1-AP (Inhibin beta A-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 17524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17524-1-AP (Inhibin beta A-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of rat brain using 17524-1-AP

  IP result of anti-Inhibin beta A-Specific (IP:17524-1-AP, 4ug; Detection:17524-1-AP 1:500) with rat brain tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, PC-3 cells, rat brain tissue
• Positive IP detected in: rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17524-1-AP targets Inhibin beta A-Specific in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: inhibin, beta A
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: NM_002192
• Gene Symbol: Inhibin beta A
• Gene ID (NCBI): 3624
• RRID: AB_2296035
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08476
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inhibin βA (INHBA) is a ligand in the transforming growth factor β (TGF-β) superfamily. Inhibin βA forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin βA can also bind the βB and α isoforms to form activin AB and inhibin, respectively.

Publications

What published studies show

Anti-INHBA (Inhibin Beta A) antibody, catalog #17524-1-AP, has 4 total citations. These appear in Cancer Cell International, Frontiers in Endocrinology, Oncology Research, and Journal of Dental Sciences. Research fields span pancreatic cancer chemoresistance via pyrimidine metabolism, polycystic ovarian syndrome with BMP4 signaling, gastric cancer progression through MAPK/ITGA6 pathway activation, and head and neck cancer cisplatin resistance involving cancer-associated fibroblast disruption.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP,IHC,IF	Cancer Cell Int; INHBA promotes chemoresistance in pancreatic cancer by enhancing CTPS1 stability and mediating pyrimidine metabolism.; Authors - Xinghao Sun ; KD Validated
rat	WB	Front Endocrinol (Lausanne); Amelioration of polycystic ovarian morphology by Tokishakuyakusan in a PCOS rat model: association with bone morphogenetic protein 4.; Authors - Mako Ueda
human	WB,IHC,IF,CoIP	Oncol Res; INHBA Promotes the Progression of Gastric Cancer by Activating MAPK Signaling Pathway via Targeting ITGA6.; Authors - Guojian Zhou ; KD Validated
human	WB	J Dent Sci; Ovatodiolide overcomes cisplatin resistance in head and neck cancer by disrupting a novel oncogenic signature and cancer-associated fibroblast activation.; Authors - Chi-Hsueh Yang