INPP5E Monoclonal antibody

• 

  WB analysis using 68602-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68602-1-Ig (INPP5E antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68602-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68602-1-Ig (INPP5E antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HaCaT using 68602-1-Ig

  HaCaT cells were subjected to SDS PAGE followed by western blot with 68602-1-Ig (INPP5E antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

INPP5E Monoclonal Antibody for WB, ELISA

Cat No. 68602-1-Ig

Clone No.2B3D6

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Synonyms

INPP5E, PPI5PIV

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HaCaT cells, HEK-293 cells, U-251 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68602-1-Ig targets INPP5E in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11959

  Product name: Recombinant human INPP5E protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC028032

  Sequence: MPSKAENLRPSEPAPQPPEGRTLQGQLPGAPPAQRAGSPPDAPGSESPALACSTPATPSGEDPPARAAPIAPRPPARPRLERALSLDDKGWRRRRFRGSQEDLEARNGTSPSRGSVQSEGPGAPAHSCSPPCLSTSLQEIPKSRGVLSSERGSPSSGGNPLSGVASSSPNLPHRDAAVAGSSPRLPSLLPPRPPPALSLDIASDSLRTANKVDSDLADYKLRAQPLLVRAHSSLGPGRPRSPLACDDCSLRSAKSSFSLLAPIRSKDVRSRSYLEGSLLASGALLGADELARYFPDRNVALFVATWNMQGQKELPPSLDEFLLPAEADYAQDLYVIGVQEGCSDRREWET

• Full Name: inositol polyphosphate-5-phosphatase, 72 kDa
• Calculated Molecular Weight: 644 aa, 70 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC028032
• Gene Symbol: INPP5E
• Gene ID (NCBI): 56623
• RRID: AB_3085298
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NRR6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INPP5E(72 kDa inositol polyphosphate 5-phosphatase) converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns-P2.In mouse, highest protein expression was in brain, heart, and testis, with lower expression in thymus and lung, and very little expression in kidney, spleen, and liver(PMID:10764818). Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1) and mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS)(PMID:19668215).